› 6l4o

X-ray diffraction
2.6Å resolution

Crystal structure of API5-FGF2 complex

Released:
DOI: 10.2210/pdb6l4o/pdb
PDB entry 6l4o coloured by chain, front view.
PDB entry 6l4o coloured by chain, side view.
PDB entry 6l4o coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Regulation of mRNA export through API5 and nuclear FGF2 interaction.
Bong SM, Bae SH, Song B, Gwak H, Yang SW, Kim S, Nam S, Rajalingam K, Oh SJ, Kim TW, Park S, Jang H, Lee BI
Nucleic Acids Res 48 6340-6352 (2020)
PMID: 32383752

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140535 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Apoptosis inhibitor 5 Chain: A
Molecule details ›
Chain: A
Length: 544 amino acids
Theoretical weight: 61.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BZZ5 (Residues: 1-524; Coverage: 100%)
Gene names: API5, MIG8
Sequence domains: Apoptosis inhibitory protein 5 (API5)
Fibroblast growth factor 2 Chain: B
Molecule details ›
Chain: B
Length: 176 amino acids
Theoretical weight: 19.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09038 (Residues: 135-288; Coverage: 54%)
Gene names: FGF2, FGFB
Sequence domains: Fibroblast growth factor

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P212121
Unit cell:
a: 46.862Å b: 76.523Å c: 208.158Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.262
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Types of nuclear localization signals and mechanisms of protein import into the nucleus.
Lu et al. (2021)
2 more

2 mentions without citation

Regulation of mRNA export through API5 and nuclear FGF2 interaction.
Bong et al. (2020)
1 more