› 6kod

X-ray diffraction
3Å resolution

Cu(II) complex of HOCl-induced flavoprotein disulfide reductase RclA C43S mutant from Escherichia coli

Released:
DOI: 10.2210/pdb6kod/pdb
PDB entry 6kod coloured by chain, front view.
PDB entry 6kod coloured by chain, side view.
PDB entry 6kod coloured by chain, top view.
Source organism: Escherichia coli BL21(DE3)
Primary publication:
Structure and function of the hypochlorous acid-induced flavoprotein RclA from Escherichia coli.
Baek Y, Kim J, Ahn J, Jo I, Hong S, Ryu S, Ha NC
J Biol Chem 295 3202-3212 (2020)
PMID: 31988242

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160132 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable pyridine nucleotide-disulfide oxidoreductase RclA Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 448 amino acids
Theoretical weight: 49.02 KDa
Source organism: Escherichia coli BL21(DE3)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P77212 (Residues: 1-441; Coverage: 100%)
Gene names: JW5040, b0304, rclA, ykgC
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand FAD 4 x FAD
Ligand CU 52 x CU
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P21
Unit cell:
a: 72.498Å b: 189.261Å c: 94.933Å
α: 90° β: 107.434° γ: 90°
R-values:
R R work R free
0.21 0.208 0.256
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Surviving Reactive Chlorine Stress: Responses of Gram-Negative Bacteria to Hypochlorous Acid.
da Cruz Nizer et al. (2020)
8 other articles

1 mention without citation

Structure and function of the hypochlorous acid-induced flavoprotein RclA from Escherichia coli.
Baek et al. (2020)