› 6kmu

X-ray diffraction
2.1Å resolution

P22/P10 complex of caspase-11 mutant C254A

Released:
DOI: 10.2210/pdb6kmu/pdb
PDB entry 6kmu coloured by chain, front view.
PDB entry 6kmu coloured by chain, side view.
PDB entry 6kmu coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural Mechanism for GSDMD Targeting by Autoprocessed Caspases in Pyroptosis.
Wang K, Sun Q, Zhong X, Zeng M, Zeng H, Shi X, Li Z, Wang Y, Zhao Q, Shao F, Ding J
Cell 180 941-955.e20 (2020)
PMID: 32109412

Function and Biology Details

Reaction catalysed: 
Strict requirement for Asp at the P1 position and has a preferred cleavage sequence of (Ile/Leu/Val/Phe)-Gly-His-Asp-|-
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159725 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Caspase-4 subunit p20 Chains: A, E
Molecule details ›
Chains: A, E
Length: 184 amino acids
Theoretical weight: 20.78 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P70343 (Residues: 102-285; Coverage: 49%)
Gene names: Casp11, Casp4, Caspl, Ich3
Sequence domains: Caspase domain
Caspase-4 subunit p10 Chains: B, F, H
Molecule details ›
Chains: B, F, H
Length: 86 amino acids
Theoretical weight: 10.11 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P70343 (Residues: 288-373; Coverage: 23%)
Gene names: Casp11, Casp4, Caspl, Ich3
Sequence domains: Caspase domain
Caspase-4 subunit p20 Chain: C
Molecule details ›
Chain: C
Length: 185 amino acids
Theoretical weight: 20.88 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P70343 (Residues: 101-285; Coverage: 50%)
Gene names: Casp11, Casp4, Caspl, Ich3
Sequence domains: Caspase domain
Caspase-4 subunit p10 Chain: D
Molecule details ›
Chain: D
Length: 87 amino acids
Theoretical weight: 10.21 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P70343 (Residues: 287-373; Coverage: 23%)
Gene names: Casp11, Casp4, Caspl, Ich3
Sequence domains: Caspase domain
Caspase-4 subunit p20 Chain: G
Molecule details ›
Chain: G
Length: 154 amino acids
Theoretical weight: 17.41 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P70343 (Residues: 101-254; Coverage: 41%)
Gene names: Casp11, Casp4, Caspl, Ich3
Sequence domains: Caspase domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NFPSS BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 61.971Å b: 102.002Å c: 99.744Å
α: 90° β: 93.52° γ: 90°
R-values:
R R work R free
0.211 0.21 0.251
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

88 review citations

Necroptosis, pyroptosis and apoptosis: an intricate game of cell death.
Bertheloot et al. (2021)
87 more

1 mention without citation

The Gasdermin D N-terminal fragment acts as a negative feedback system to inhibit inflammasome-mediated activation of Caspase-1/11.
Hu et al. (2022)