Function and Biology Details
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero tetramer (preferred)
Assembly name:
HigAB toxin-antitoxin complex (preferred)
PDBe Complex ID:
PDB-CPX-159141 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
mRNA interferase toxin HigB
Molecule details ›
Chains: A, C
Length: 118 amino acids
Theoretical weight: 13.68 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: HigB_toxin, RelE-like toxic component of a toxin-antitoxin system
Length: 118 amino acids
Theoretical weight: 13.68 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P64578 (Residues: 1-104; Coverage: 100%)
Sequence domains: HigB_toxin, RelE-like toxic component of a toxin-antitoxin system
Antitoxin HigA
Molecule details ›
Chains: B, D
Length: 138 amino acids
Theoretical weight: 15.01 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Helix-turn-helix
Length: 138 amino acids
Theoretical weight: 15.01 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P67701 (Residues: 1-138; Coverage: 100%)
Sequence domains: Helix-turn-helix
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
BESSY BEAMLINE 14.2
Spacegroup:
P21
Expression system: Escherichia coli BL21(DE3)
