› 6kmb

X-ray diffraction
2.4Å resolution

Crystal structure of Sth1 bromodomain

Released:
DOI: 10.2210/pdb6kmb/pdb
PDB entry 6kmb coloured by chain, front view.
PDB entry 6kmb coloured by chain, side view.
PDB entry 6kmb coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
The Structural Basis for Specific Recognition of H3K14 Acetylation by Sth1 in the RSC Chromatin Remodeling Complex.
Chen G, Li W, Yan F, Wang D, Chen Y
Structure 28 111-118.e3 (2020)
PMID: 31711754

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152385 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nuclear protein STH1/NPS1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 112 amino acids
Theoretical weight: 13.33 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P32597 (Residues: 1248-1359; Coverage: 8%)
Gene names: NPS1, STH1, YIL126W
Sequence domains: Bromodomain

Ligands and Environments

1 bound ligand:
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P61
Unit cell:
a: 108.855Å b: 108.855Å c: 110.928Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.198 0.242
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Structure and Function of Chromatin Remodelers.
Reyes et al. (2021)
2 more

1 mention without citation

Catching Nucleosome by Its Decorated Tails Determines Its Functional States.
Sehrawat et al. (2022)