› 6kfz

X-ray diffraction
1.96Å resolution

SufS from Bacillus subtilis, soaked with L-cysteine for 90 sec at 1.96 angstrom resolution

Released:
DOI: 10.2210/pdb6kfz/pdb
PDB entry 6kfz coloured by chain, front view.
PDB entry 6kfz coloured by chain, side view.
PDB entry 6kfz coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Snapshots of PLP-substrate and PLP-product external aldimines as intermediates in two types of cysteine desulfurase enzymes.
Nakamura R, Hikita M, Ogawa S, Takahashi Y, Fujishiro T
FEBS J 287 1138-1154 (2020)
PMID: 31587510

Function and Biology Details

Reaction catalysed: 
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128426 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine desulfurase SufS Chain: A
Molecule details ›
Chain: A
Length: 419 amino acids
Theoretical weight: 46.62 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O32164 (Residues: 1-406; Coverage: 100%)
Gene names: BSU32690, csd, sufS, yurW
Sequence domains: Aminotransferase class-V

Ligands and Environments

2 bound ligands:
Ligand C6P 1 x C6P
Ligand PEG 3 x PEG
1 modified residue:
Ligand CSS 1 x CSS

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P3121
Unit cell:
a: 92.8Å b: 92.8Å c: 128.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.157 0.156 0.177
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Fe-S cluster biogenesis by the bacterial Suf pathway.
Blahut et al. (2020)
3 more

2 mentions without citation

Snapshots of PLP-substrate and PLP-product external aldimines as intermediates in two types of cysteine desulfurase enzymes.
Nakamura et al. (2020)
1 more