6j56

X-ray diffraction
1.8Å resolution

Crystal structure of Myosin VI CBD in complex with Tom1 MBM

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-130090 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Unconventional myosin-VI Chains: A, B
Molecule details ›
Chains: A, B
Length: 129 amino acids
Theoretical weight: 15.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q9UM54 (Residues: 1166-1294; Coverage: 10%)
Gene names: KIAA0389, MYO6
Sequence domains: Myosin VI cargo binding domain
Target of Myb1 membrane trafficking protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 27 amino acids
Theoretical weight: 2.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: O60784 (Residues: 437-441, 442-462; Coverage: 5%)
Gene name: TOM1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 40.733Å b: 74.875Å c: 50.873Å
α: 90° β: 106.34° γ: 90°
R-values:
R R work R free
0.179 0.177 0.234
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'