› 6j1y

X-ray diffraction
2.55Å resolution

Semi-open conformation E3 ligase

Released:
DOI: 10.2210/pdb6j1y/pdb
PDB entry 6j1y coloured by chain, front view.
PDB entry 6j1y coloured by chain, side view.
PDB entry 6j1y coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A multi-lock inhibitory mechanism for fine-tuning enzyme activities of the HECT family E3 ligases.
Wang Z, Liu Z, Chen X, Li J, Yao W, Huang S, Gu A, Lei QY, Mao Y, Wen W
Nat Commun 10 3162 (2019)
PMID: 31320636

Function and Biology Details

Reaction catalysed: 
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190482 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NEDD4-like E3 ubiquitin-protein ligase WWP1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 519 amino acids
Theoretical weight: 61.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4
UniProt:
  • Canonical: Q9H0M0 (Residues: 410-922; Coverage: 56%)
Gene name: WWP1
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P1
Unit cell:
a: 60.019Å b: 59.37Å c: 85.075Å
α: 99.39° β: 92.29° γ: 108.75°
R-values:
R R work R free
0.21 0.208 0.254
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4

Quick links

Citations

10 review citations

The many substrates and functions of NEDD4-1.
Huang et al. (2019)
9 more

2 mentions without citation

Huntingtin Ubiquitination Mechanisms and Novel Possible Therapies to Decrease the Toxic Effects of Mutated Huntingtin.
Fiorillo et al. (2021)
1 more