› 6ij5

X-ray diffraction
1.72Å resolution

Crystal structure of PETase P181A mutant from Ideonella sakaiensis

Released:
DOI: 10.2210/pdb6ij5/pdb
PDB entry 6ij5 coloured by chain, front view.
PDB entry 6ij5 coloured by chain, side view.
PDB entry 6ij5 coloured by chain, top view.
Source organism: Ideonella sakaiensis
Primary publication:
Rational Protein Engineering of Thermo-Stable PETase from Ideonella sakaiensis for Highly Efficient PET Degradation
Son HF, Cho IJ, Joo S, Seo H, Sagong HY, Choi SY, Lee SY, Kim KJ
ACS Catalysis 9 3519-3526 (2019)

Function and Biology Details

Reaction catalysed: 
(Ethylene terephthalate)(n) + H(2)O = (ethylene terephthalate)(n-1) + ethylene terephthalate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102331 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly(ethylene terephthalate) hydrolase Chain: A
Molecule details ›
Chain: A
Length: 300 amino acids
Theoretical weight: 31.49 KDa
Source organism: Ideonella sakaiensis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0K8P6T7 (Residues: 34-290; Coverage: 98%)
Gene name: ISF6_4831
Sequence domains: Dienelactone hydrolase family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: C2
Unit cell:
a: 115.479Å b: 50.937Å c: 41.262Å
α: 90° β: 92.58° γ: 90°
R-values:
R R work R free
0.209 0.206 0.262
Expression system: Escherichia coli

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