6iie

X-ray diffraction
2.14Å resolution

Crystal structure of human diacylglycerol kinase alpha EF-hand domains bound to Ca2+

Released:

Function and Biology Details

Reactions catalysed:
ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate
ATP + 1-O-alkyl-sn-glycerol = ADP + 1-O-alkyl-sn-glycerol 3-phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-150056 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Diacylglycerol kinase alpha Chain: A
Molecule details ›
Chain: A
Length: 93 amino acids
Theoretical weight: 10.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P23743 (Residues: 107-197; Coverage: 12%)
Gene names: DAGK, DAGK1, DGKA

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P3221
Unit cell:
a: 58.19Å b: 58.19Å c: 61.12Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.185 0.238
Expression system: Escherichia coli