› 6ib7

X-ray diffraction
2.24Å resolution

Structure of wild type SuhB

Released:
DOI: 10.2210/pdb6ib7/pdb
PDB entry 6ib7 coloured by chain, front view.
PDB entry 6ib7 coloured by chain, side view.
PDB entry 6ib7 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis for the function of SuhB as a transcription factor in ribosomal RNA synthesis.
Huang YH, Said N, Loll B, Wahl MC
Nucleic Acids Res 47 6488-6503 (2019)
PMID: 31020314

Function and Biology Details

Reaction catalysed: 
Myo-inositol phosphate + H(2)O = myo-inositol + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142357 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nus factor SuhB Chain: A
Molecule details ›
Chain: A
Length: 271 amino acids
Theoretical weight: 29.54 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ADG4 (Residues: 1-267; Coverage: 100%)
Gene names: JW2517, b2533, ssyA, suhB
Sequence domains: Inositol monophosphatase family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: C2
Unit cell:
a: 90.718Å b: 46.031Å c: 72.915Å
α: 90° β: 125.4° γ: 90°
R-values:
R R work R free
0.217 0.215 0.26
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Emerging Quantitative Biochemical, Structural, and Biophysical Methods for Studying Ribosome and Protein-RNA Complex Assembly.
Gor et al. (2023)
7 other articles

1 mention without citation

Structural basis for the function of SuhB as a transcription factor in ribosomal RNA synthesis.
Huang et al. (2019)