6i8a

X-ray diffraction
2.65Å resolution

The crystal structure of the Pol2 catalytic domain of DNA polymerase epsilon carrying a P301R substitution.

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-120005 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase epsilon catalytic subunit A Chains: A, B
Molecule details ›
Chains: A, B
Length: 1190 amino acids
Theoretical weight: 137.13 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P21951 (Residues: 1-1185; Coverage: 53%)
Gene names: DUN2, N0825, POL2, YNL262W
Sequence domains:
Primer DNA Chains: C, P
Molecule details ›
Chains: C, P
Length: 11 nucleotides
Theoretical weight: 3.29 KDa
Source organism: synthetic construct
Expression system: Not provided
Template DNA Chains: D, T
Molecule details ›
Chains: D, T
Length: 16 nucleotides
Theoretical weight: 4.89 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P2
Unit cell:
a: 154.466Å b: 70.257Å c: 159.335Å
α: 90° β: 112.85° γ: 90°
R-values:
R R work R free
0.232 0.229 0.279
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided