› 6i6m

X-ray diffraction
1.2Å resolution

Function and Biology Details

Reactions catalysed: 
S-adenosyl-L-methionine + (S)-scoulerine = S-adenosyl-L-homocysteine + (S)-tetrahydrocolumbamine
S-adenosyl-L-methionine + 5,8,13,13a-tetrahydrocolumbamine = S-adenosyl-L-homocysteine + tetrahydropalmatine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125094 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Scoulerine-9-O-methyltransferase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 393 amino acids
Theoretical weight: 42.73 KDa
Source organism: Papaver somniferum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: I3V6A7 (Residues: 1-390; Coverage: 100%)
Gene names: PSMT1, SOMT1
Sequence domains:
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH
1 bound ligand:
Ligand SLX 2 x SLX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P21
Unit cell:
a: 68.868Å b: 76.617Å c: 77.158Å
α: 90° β: 101.55° γ: 90°
R-values:
R R work R free
0.117 0.117 0.13
Expression system: Escherichia coli BL21(DE3)

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