› 6hut

X-ray diffraction
1.29Å resolution

GFP8 - a stabilized variant of cycle-3 GFP

Released:
DOI: 10.2210/pdb6hut/pdb
PDB entry 6hut coloured by chain, front view.
PDB entry 6hut coloured by chain, side view.
PDB entry 6hut coloured by chain, top view.
Source organism: Aequorea victoria
Entry authors: Petek M, Rangel Pereira M

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 28.41 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 52.16Å b: 63.37Å c: 69.21Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.186 0.203
Expression system: Escherichia coli BL21(DE3)

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