› 6hpt

X-ray diffraction
1.44Å resolution

Crystal structure of human Pif1 helicase, apoform.

Released:
DOI: 10.2210/pdb6hpt/pdb
PDB entry 6hpt coloured by chain, front view.
PDB entry 6hpt coloured by chain, side view.
PDB entry 6hpt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and functional analysis of the nucleotide and DNA binding activities of the human PIF1 helicase.
Dehghani-Tafti S, Levdikov V, Antson AA, Bax B, Sanders CM
Nucleic Acids Res 47 3208-3222 (2019)
PMID: 30698796

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190630 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent DNA helicase PIF1 Chain: A
Molecule details ›
Chain: A
Length: 439 amino acids
Theoretical weight: 47.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H611 (Residues: 206-641; Coverage: 68%)
Gene names: C15orf20, PIF1
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 63.54Å b: 81.4Å c: 91.48Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.137 0.134 0.195
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

History of DNA Helicases.
Brosh et al. (2020)
5 more

1 mention without citation

Structural and functional analysis of the nucleotide and DNA binding activities of the human PIF1 helicase.
Dehghani-Tafti et al. (2019)