› 6hpc

X-ray diffraction
2.26Å resolution

Crystal structure of the HicB antitoxin from E. coli

Released:
DOI: 10.2210/pdb6hpc/pdb
PDB entry 6hpc coloured by chain, front view.
PDB entry 6hpc coloured by chain, side view.
PDB entry 6hpc coloured by chain, top view.
Source organism: Escherichia coli 2-210-07_S3_C3
Primary publication:
The E. coli HicB Antitoxin Contains a Structurally Stable Helix-Turn-Helix DNA Binding Domain.
Manav MC, Turnbull KJ, Jurėnas D, Garcia-Pino A, Gerdes K, Brodersen DE
Structure 27 1675-1685.e3 (2019)
PMID: 31495532

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159221 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Antitoxin HicB Chains: A, B
Molecule details ›
Chains: A, B
Length: 138 amino acids
Theoretical weight: 15.45 KDa
Source organism: Escherichia coli 2-210-07_S3_C3
Expression system: Escherichia coli
UniProt:
  • Canonical: P67697 (Residues: 1-138; Coverage: 100%)
Gene names: JW1433, b1438, hicB, ydcQ
Sequence domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: P21
Unit cell:
a: 43.09Å b: 60.38Å c: 59.34Å
α: 90° β: 103.76° γ: 90°
R-values:
R R work R free
0.227 0.227 0.268
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Prokaryote toxin-antitoxin modules: Complex regulation of an unclear function.
De Bruyn et al. (2021)
2 more