› 6hkp

X-ray diffraction
1.9Å resolution

Factor Inhibiting HIF (FIH) in complex with zinc, NOG and ASPP2 (970-992)

Released:
DOI: 10.2210/pdb6hkp/pdb
PDB entry 6hkp coloured by chain, front view.
PDB entry 6hkp coloured by chain, side view.
PDB entry 6hkp coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Leissing TM, Clifton IJ, Lu X, Schofield CJ

Function and Biology Details

Reactions catalysed: 
Hypoxia-inducible factor-L-asparagine + 2-oxoglutarate + O(2) = hypoxia-inducible factor-(3S)-3-hydroxy-L-asparagine + succinate + CO(2)
[Ankyrin-repeat domain protein]-L-asparagine + 2-oxoglutarate + O(2) = [ankyrin-repeat domain protein]-(3S)-3-hydroxy-L-asparagine + succinate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171788 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hypoxia-inducible factor 1-alpha inhibitor Chain: A
Molecule details ›
Chain: A
Length: 350 amino acids
Theoretical weight: 40.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NWT6 (Residues: 1-349; Coverage: 100%)
Gene names: FIH1, HIF1AN
Sequence domains: Cupin-like domain
Apoptosis-stimulating of p53 protein 2 Chain: S
Molecule details ›
Chain: S
Length: 23 amino acids
Theoretical weight: 2.42 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q13625 (Residues: 970-992; Coverage: 2%)
Gene names: ASPP2, BBP, TP53BP2
Sequence domains: Ankyrin repeat

Ligands and Environments

4 bound ligands:
Ligand SO4 6 x SO4
Ligand GOL 5 x GOL
Ligand OGA 1 x OGA
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P41212
Unit cell:
a: 86.02Å b: 86.02Å c: 148.508Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.176 0.195
Expression systems:
  • Escherichia coli
  • Not provided

Quick links