› 6hji

X-ray diffraction
2.8Å resolution

Xray structure of GLIC in complex with crotonate

Released:
DOI: 10.2210/pdb6hji/pdb
PDB entry 6hji coloured by chain, front view.
PDB entry 6hji coloured by chain, side view.
PDB entry 6hji coloured by chain, top view.
Source organism: Gloeobacter violaceus
Primary publication:
Structural evidence for the binding of monocarboxylates and dicarboxylates at pharmacologically relevant extracellular sites of a pentameric ligand-gated ion channel.
Fourati Z, Sauguet L, Delarue M
Acta Crystallogr D Struct Biol 76 668-675 (2020)
PMID: 32627739

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181806 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proton-gated ion channel Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 317 amino acids
Theoretical weight: 36.28 KDa
Source organism: Gloeobacter violaceus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7NDN8 (Residues: 43-359; Coverage: 100%)
Gene names: glr4197, glvI
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain

Ligands and Environments

5 bound ligands:
Ligand PLC 15 x PLC
Ligand CL 12 x CL
Ligand NA 6 x NA
Ligand LMT 6 x LMT
Ligand BEO 5 x BEO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 180.943Å b: 133.242Å c: 159.88Å
α: 90° β: 101.96° γ: 90°
R-values:
R R work R free
0.19 0.189 0.204
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Dynamic closed states of a ligand-gated ion channel captured by cryo-EM and simulations.
Rovšnik et al. (2021)

1 mention without citation

Structural evidence for the binding of monocarboxylates and dicarboxylates at pharmacologically relevant extracellular sites of a pentameric ligand-gated ion channel.
Fourati et al. (2020)