› 6gya

X-ray diffraction
2.95Å resolution

Amylase in complex with branched ligand

Released:
DOI: 10.2210/pdb6gya/pdb
PDB entry 6gya coloured by chain, front view.
PDB entry 6gya coloured by chain, side view.
PDB entry 6gya coloured by chain, top view.
Source organism: Alicyclobacillus sp.
Primary publication:
The structure of the AliC GH13 α-amylase from Alicyclobacillus sp. reveals the accommodation of starch branching points in the α-amylase family.
Agirre J, Moroz O, Meier S, Brask J, Munch A, Hoff T, Andersen C, Wilson KS, Davies GJ
Acta Crystallogr D Struct Biol 75 1-7 (2019)
PMID: 30644839

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104924 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Glycosyl hydrolase family 13 catalytic domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 484 amino acids
Theoretical weight: 53.98 KDa
Source organism: Alicyclobacillus sp.
Expression system: Bacillus subtilis
UniProt:
  • Canonical: A0A3P8MUS3 (Residues: 29-514; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
4 bound ligands:
Ligand CA 12 x CA
Ligand NA 4 x NA
Ligand GLC 4 x GLC
Ligand BGC 3 x BGC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P61
Unit cell:
a: 212.18Å b: 212.18Å c: 172.22Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.157 0.156 0.183
Expression system: Bacillus subtilis

Quick links

Citations

1 review citation

Structural and functional adaptation in extremophilic microbial α-amylases.
Ahmad et al. (2022)
4 other articles

1 mention without citation

The structure of the AliC GH13 α-amylase from Alicyclobacillus sp. reveals the accommodation of starch branching points in the α-amylase family.
Agirre et al. (2019)