› 6gxd

X-ray diffraction
1.8Å resolution

The hit-and-return system enables efficient time-resolved serial synchrotron crystallography: FAcD752MS after reaction initiation

Released:
DOI: 10.2210/pdb6gxd/pdb
PDB entry 6gxd coloured by chain, front view.
PDB entry 6gxd coloured by chain, side view.
PDB entry 6gxd coloured by chain, top view.
Source organism: Rhodopseudomonas palustris
Primary publication:
The hit-and-return system enables efficient time-resolved serial synchrotron crystallography.
Schulz EC, Mehrabi P, Müller-Werkmeister HM, Tellkamp F, Jha A, Stuart W, Persch E, De Gasparo R, Diederich F, Pai EF, Miller RJD
Nat Methods 15 901-904 (2018)
PMID: 30377366

Function and Biology Details

Reaction catalysed: 
Haloacetate + H(2)O = glycolate + halide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179995 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluoroacetate dehalogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 306 amino acids
Theoretical weight: 34.07 KDa
Source organism: Rhodopseudomonas palustris
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6NAM1 (Residues: 1-302; Coverage: 100%)
Gene name: RPA1163
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand FAH 1 x FAH
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: P21
Unit cell:
a: 41.37Å b: 78.54Å c: 83.51Å
α: 90° β: 102.76° γ: 90°
R-values:
R R work R free
0.167 0.165 0.209
Expression system: Escherichia coli

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Citations

6 review citations

Using photocaging for fast time-resolved structural biology studies.
Monteiro et al. (2021)
5 more