› 6gwc

X-ray diffraction
2.6Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112167 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ALPHA-TUBULIN Chain: A
Molecule details ›
Chain: A
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Ovis aries
Structure domains:
Tubulin beta chain Chain: B
Molecule details ›
Chain: B
Length: 445 amino acids
Theoretical weight: 50 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: D0VWY9 (Residues: 1-445; Coverage: 100%)
Sequence domains:
Structure domains:
iE5 ALPHAREP Chain: C
Molecule details ›
Chain: C
Length: 232 amino acids
Theoretical weight: 25.17 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

4 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 2 x MG
Ligand PGE 1 x PGE
Ligand MES 2 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P3221
Unit cell:
a: 102.346Å b: 102.346Å c: 216.207Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.173 0.223
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The molecular biology of tubulinopathies: Understanding the impact of variants on tubulin structure and microtubule regulation.
Hoff et al. (2022)
4 other articles

6 mentions without citation

The Mechanism of Tubulin Assembly into Microtubules: Insights from Structural Studies.
Knossow et al. (2020)
5 more