› 6gqd

X-ray diffraction
1.52Å resolution

Structure of human galactose-1-phosphate uridylyltransferase (GALT), with crystallization epitope mutations A21Y:A22T:T23P:R25L

Released:
DOI: 10.2210/pdb6gqd/pdb
PDB entry 6gqd coloured by chain, front view.
PDB entry 6gqd coloured by chain, side view.
PDB entry 6gqd coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Fairhead M, Strain-Damerell C, Kopec J, Bezerra GA, Zhang M, Burgess-Brown N, von Delft F, Arrowsmith C, Edwards A, Bountra C, Yue WW, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139830 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Galactose-1-phosphate uridylyltransferase Chain: A
Molecule details ›
Chain: A
Length: 380 amino acids
Theoretical weight: 43.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P07902 (Residues: 1-379; Coverage: 100%)
Gene name: GALT
Sequence domains:
Structure domains: HIT-like

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand EDO 2 x EDO
Ligand H2U 1 x H2U
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P21212
Unit cell:
a: 72.647Å b: 96.748Å c: 55.426Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.162 0.188
Expression system: Escherichia coli

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