› 6gpu

X-ray diffraction
1.17Å resolution

Crystal structure of miniSOG at 1.17A resolution

Released:
DOI: 10.2210/pdb6gpu/pdb
PDB entry 6gpu coloured by chain, front view.
PDB entry 6gpu coloured by chain, side view.
PDB entry 6gpu coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Tailing miniSOG: structural bases of the complex photophysics of a flavin-binding singlet oxygen photosensitizing protein.
Torra J, Lafaye C, Signor L, Aumonier S, Flors C, Shu X, Nonell S, Gotthard G, Royant A
Sci Rep 9 2428 (2019)
PMID: 30787421

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161064 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phototropin-2 Chain: A
Molecule details ›
Chain: A
Length: 115 amino acids
Theoretical weight: 13.32 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P93025 (Residues: 387-492; Coverage: 12%)
Gene names: At5g58140, CAV1, K21L19.6, KIN7, NPL1, PHOT2
Sequence domains: PAS domain
Structure domains: PAS domain

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN
4 bound ligands:
Ligand CL 1 x CL
Ligand MG 1 x MG
Ligand CO 1 x CO
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P43212
Unit cell:
a: 40.02Å b: 40.02Å c: 134.29Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.134 0.133 0.164
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Nanotechnological Applications Based on Bacterial Encapsulins.
Rodríguez et al. (2021)
1 more

4 mentions without citation

Genetically encoded tools for measuring and manipulating metabolism.
Choe et al. (2022)
3 more