› 6gp0

X-ray diffraction
1.5Å resolution

Structure of mEos4b in the red fluorescent state

Released:
DOI: 10.2210/pdb6gp0/pdb
PDB entry 6gp0 coloured by chain, front view.
PDB entry 6gp0 coloured by chain, side view.
PDB entry 6gp0 coloured by chain, top view.
Source organism: Lobophyllia hemprichii
Primary publication:
Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM.
De Zitter E, Thédié D, Mönkemöller V, Hugelier S, Beaudouin J, Adam V, Byrdin M, Van Meervelt L, Dedecker P, Bourgeois D
Nat Methods 16 707-710 (2019)
PMID: 31285624

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177805 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green to red photoconvertible GFP-like protein EosFP Chain: A
Molecule details ›
Chain: A
Length: 257 amino acids
Theoretical weight: 29.55 KDa
Source organism: Lobophyllia hemprichii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5S6Z9 (Residues: 1-226; Coverage: 100%)
Sequence domains: Green fluorescent protein

Ligands and Environments

No bound ligands
3 modified residues:
Ligand NFA 1 x NFA
Ligand 5SQ 1 x 5SQ
Ligand RC7 1 x RC7

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 38.61Å b: 57.9Å c: 102.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.154 0.19
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Advanced imaging and labelling methods to decipher brain cell organization and function.
Choquet et al. (2021)
2 more

1 mention without citation

Xtrapol8 enables automatic elucidation of low-occupancy intermediate-states in crystallographic studies.
De Zitter et al. (2022)