› 6gl2

X-ray diffraction
1.96Å resolution

Structure of ZgEngAGH5_4 wild type at 1.2 Angstrom resolution

Released:
DOI: 10.2210/pdb6gl2/pdb
PDB entry 6gl2 coloured by chain, front view.
PDB entry 6gl2 coloured by chain, side view.
PDB entry 6gl2 coloured by chain, top view.
Source organism: Zobellia galactanivorans
Primary publication:
The laterally acquired GH5 ZgEngAGH5_4 from the marine bacterium Zobellia galactanivorans is dedicated to hemicellulose hydrolysis.
Dorival J, Ruppert S, Gunnoo M, Orłowski A, Chapelais-Baron M, Dabin J, Labourel A, Thompson D, Michel G, Czjzek M, Genicot S
Biochem J 475 3609-3628 (2018)
PMID: 30341165

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123850 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycoside hydrolase family 5 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 337 amino acids
Theoretical weight: 38.44 KDa
Source organism: Zobellia galactanivorans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G0L8Z3 (Residues: 55-385; Coverage: 83%)
Gene names: engA, zobellia_208
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

1 bound ligand:
Ligand IMD 2 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 55.49Å b: 48.46Å c: 59.26Å
α: 90° β: 104.35° γ: 90°
R-values:
R R work R free
0.173 0.172 0.2
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Multifunctional cellulases are potent, versatile tools for a renewable bioeconomy.
Glasgow et al. (2021)
4 other articles

1 mention without citation

In silico Structural, Functional and Phylogenetic Analyses of cellulase from Ruminococcus albus.
Hoda et al. (2021)