6g8q

X-ray diffraction
1.85Å resolution

14-3-3sigma in complex with a A130beta3A and Q133beta3Q mutated YAP pS127 phosphopeptide

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-152127 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein sigma Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P31947 (Residues: 1-231; Coverage: 93%)
Gene names: HME1, SFN
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
Transcriptional coactivator YAP1 Chain: P
Molecule details ›
Chain: P
Length: 11 amino acids
Theoretical weight: 1.19 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P46937 (Residues: 124-132; Coverage: 2%)
Gene names: YAP1, YAP65

Ligands and Environments

3 bound ligands:
3 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: C2221
Unit cell:
a: 82.108Å b: 111.9Å c: 62.62Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.146 0.144 0.175
Expression systems:
  • Escherichia coli
  • Not provided