› 6g3t

X-ray diffraction
2.53Å resolution

Function and Biology Details

Reactions catalysed: 
(1a) S-adenosyl-L-methionine + a [histone H3]-L-lysine(4) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(4)
(1a) S-adenosyl-L-methionine + a [histone H3]-L-lysine(27) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(27)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189853 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histone-lysine N-methyltransferase NSD3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 153 amino acids
Theoretical weight: 17.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BZ95 (Residues: 247-398; Coverage: 11%)
Gene names: DC28, NSD3, WHSC1L1
Sequence domains: PWWP domain

Ligands and Environments

1 bound ligand:
Ligand EPE 3 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 45.178Å b: 88.091Å c: 79.988Å
α: 90° β: 89.88° γ: 90°
R-values:
R R work R free
0.231 0.229 0.274
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Epigenetics and beyond: targeting writers of protein lysine methylation to treat disease.
Bhat et al. (2021)
13 more