› 6g1o

X-ray diffraction
1.88Å resolution

Structure of Pseudomonas aeruginosa Isocitrate Lyase, ICL

Released:
DOI: 10.2210/pdb6g1o/pdb
PDB entry 6g1o coloured by chain, front view.
PDB entry 6g1o coloured by chain, side view.
PDB entry 6g1o coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Gluconeogenic precursor availability regulates flux through the glyoxylate shunt in Pseudomonas aeruginosa.
Crousilles A, Dolan SK, Brear P, Chirgadze DY, Welch M
J Biol Chem 293 14260-14269 (2018)
PMID: 30030382

Function and Biology Details

Reaction catalysed: 
Isocitrate = succinate + glyoxylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191237 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isocitrate lyase Chain: A
Molecule details ›
Chain: A
Length: 486 amino acids
Theoretical weight: 54.07 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I0K4 (Residues: 1-486; Coverage: 92%)
Gene name: PA2634
Sequence domains: Isocitrate lyase family

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand GLV 1 x GLV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: I222
Unit cell:
a: 80.943Å b: 116.023Å c: 128.533Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.206
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Airway immunometabolites fuel Pseudomonas aeruginosa infection.
Riquelme et al. (2020)
2 more

1 mention without citation

Gluconeogenic precursor availability regulates flux through the glyoxylate shunt in Pseudomonas aeruginosa.
Crousilles et al. (2018)