› 6fgo

X-ray diffraction
2.5Å resolution

Fc in complex with engineered calcium binding domain Z

Released:
DOI: 10.2210/pdb6fgo/pdb
PDB entry 6fgo coloured by chain, front view.
PDB entry 6fgo coloured by chain, side view.
PDB entry 6fgo coloured by chain, top view.
Source organisms:
Primary publication:
Protein Engineering Allows for Mild Affinity-based Elution of Therapeutic Antibodies.
Kanje S, Venskutonytė R, Scheffel J, Nilvebrant J, Lindkvist-Petersson K, Hober S
J Mol Biol 430 3427-3438 (2018)
PMID: 29886013

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102752 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (5 distinct):
Immunoglobulin gamma-1 heavy chain Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 210 amino acids
Theoretical weight: 23.82 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P0DOX5 (Residues: 239-448; Coverage: 47%)
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Protein A Ig-binding domain-containing protein Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 69 amino acids
Theoretical weight: 7.67 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0U3TLJ6 (Residues: 43-69; Coverage: 38%)
Sequence domains: B domain
Structure domains: Immunoglobulin FC, subunit C

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
4 bound ligands:
Ligand PEG 5 x PEG
Ligand GOL 15 x GOL
Ligand CA 4 x CA
Ligand CL 9 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21212
Unit cell:
a: 90.229Å b: 120.296Å c: 126.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.204 0.232
Expression systems:
  • Cricetulus griseus
  • Escherichia coli

Quick links

Citations

3 review citations

Protein aggregation and immunogenicity of biotherapeutics.
Pham et al. (2020)
2 more