› 6fdp

Solution NMR

NMR structure of the second TPR domain of the human RPAP3 protein in complex with HSP90 peptide DTSRMEEVD

Released:
DOI: 10.2210/pdb6fdp/pdb
PDB entry 6fdp coloured by chain, ensemble of 20 models, front view.
PDB entry 6fdp coloured by chain, ensemble of 20 models, side view.
PDB entry 6fdp coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Deep Structural Analysis of RPAP3 and PIH1D1, Two Components of the HSP90 Co-chaperone R2TP Complex.
Henri J, Chagot ME, Bourguet M, Abel Y, Terral G, Maurizy C, Aigueperse C, Georgescauld F, Vandermoere F, Saint-Fort R, Behm-Ansmant I, Charpentier B, Pradet-Balade B, Verheggen C, Bertrand E, Meyer P, Cianférani S, Manival X, Quinternet M
Structure 26 1196-1209.e8 (2018)
PMID: 30033218

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139822 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RNA polymerase II-associated protein 3 Chain: A
Molecule details ›
Chain: A
Length: 120 amino acids
Theoretical weight: 13.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H6T3 (Residues: 281-395; Coverage: 17%)
Gene name: RPAP3
Sequence domains: Tetratricopeptide repeat
Structure domains: Tetratricopeptide repeat domain
Heat shock protein HSP 90-alpha Chain: B
Molecule details ›
Chain: B
Length: 9 amino acids
Theoretical weight: 1.08 KDa
Source organism: Homo sapiens
Expression system: synthetic construct
UniProt:
  • Canonical: P07900 (Residues: 724-732; Coverage: 1%)
Gene names: HSP90A, HSP90AA1, HSPC1, HSPCA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 91%
Refinement method: molecular dynamics
Expression systems:
  • Escherichia coli BL21(DE3)
  • synthetic construct

Quick links

Citations

7 review citations

Role of the Novel Hsp90 Co-Chaperones in Dynein Arms' Preassembly.
Fabczak et al. (2019)
6 more