› 6fct

X-ray diffraction
1.89Å resolution

Catalytic subunit HisG from Psychrobacter arcticus ATP phosphoribosyltransferase (HisZG ATPPRT) in complex with PRPP and ATP

Released:
DOI: 10.2210/pdb6fct/pdb
PDB entry 6fct coloured by chain, front view.
PDB entry 6fct coloured by chain, side view.
PDB entry 6fct coloured by chain, top view.
Source organism: Psychrobacter arcticus 273-4
Primary publication:
Catalytic and Anticatalytic Snapshots of a Short-Form ATP Phosphoribosyltransferase
Alphey MS, Fisher G, Hirschi JS, Stroek R, Ge Y, Gould ER, Czekster CM, Liu H, Florence GJ, Vetticatt MJ, Naismith JH, da Silva RG
ACS Catalysis (2018)

Function and Biology Details

Reaction catalysed: 
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = ATP + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175513 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP phosphoribosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 232 amino acids
Theoretical weight: 25.24 KDa
Source organism: Psychrobacter arcticus 273-4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4FQF7 (Residues: 1-231; Coverage: 100%)
Gene names: Psyc_1903, hisG
Sequence domains: ATP phosphoribosyltransferase
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

3 bound ligands:
Ligand PRP 1 x PRP
Ligand ATP 1 x ATP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Unit cell:
a: 70.58Å b: 33.93Å c: 92.391Å
α: 90° β: 104.86° γ: 90°
R-values:
R R work R free
0.182 0.18 0.217
Expression system: Escherichia coli BL21(DE3)

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