6fc2

X-ray diffraction
1.92Å resolution

Crystal structure of the eIF4E-Eap1p complex from Saccharomyces cerevisiae

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-139462 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Eukaryotic translation initiation factor 4E Chains: A, C
Molecule details ›
Chains: A, C
Length: 183 amino acids
Theoretical weight: 20.81 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07260 (Residues: 35-213; Coverage: 84%)
Gene names: CDC33, TIF45, YOL139C
Sequence domains: Eukaryotic initiation factor 4E
Protein EAP1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 64 amino acids
Theoretical weight: 7.59 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P36041 (Residues: 91-150; Coverage: 10%)
Gene names: EAP1, YKL204W

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21212
Unit cell:
a: 87.574Å b: 95.116Å c: 56.009Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.174 0.206
Expression system: Escherichia coli BL21(DE3)