› 6f9g

X-ray diffraction
2.39Å resolution

Ligand binding domain of P. putida KT2440 polyamine chemorecpetors McpU in complex putrescine.

Released:
DOI: 10.2210/pdb6f9g/pdb
PDB entry 6f9g coloured by chain, front view.
PDB entry 6f9g coloured by chain, side view.
PDB entry 6f9g coloured by chain, top view.
Source organism: Pseudomonas putida KT2440
Primary publication:
Structural Basis for Polyamine Binding at the dCACHE Domain of the McpU Chemoreceptor from Pseudomonas putida.
Gavira JA, Ortega Á, Martín-Mora D, Conejero-Muriel MT, Corral-Lugo A, Morel B, Matilla MA, Krell T
J Mol Biol 430 1950-1963 (2018)
PMID: 29758259

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183503 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyl-accepting chemotaxis protein McpU Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 321 amino acids
Theoretical weight: 34.73 KDa
Source organism: Pseudomonas putida KT2440
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q88NI1 (Residues: 14-14, 33-334; Coverage: 44%)
Gene names: PP_1228, mcpU

Ligands and Environments

3 bound ligands:
Ligand PUT 5 x PUT
Ligand GOL 5 x GOL
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2221
Unit cell:
a: 133.984Å b: 164.521Å c: 123.612Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.234
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Functional Annotation of Bacterial Signal Transduction Systems: Progress and Challenges.
Martín-Mora et al. (2018)
2 more

4 mentions without citation

Chemotaxis of Beneficial Rhizobacteria to Root Exudates: The First Step towards Root-Microbe Rhizosphere Interactions.
Feng et al. (2021)
3 more