› 6f75

X-ray diffraction
1.89Å resolution

Carboxypeptidase T mutant L254N with Sulphamoil Leucine

Released:
DOI: 10.2210/pdb6f75/pdb
PDB entry 6f75 coloured by chain, front view.
PDB entry 6f75 coloured by chain, side view.
PDB entry 6f75 coloured by chain, top view.
Source organism: Thermoactinomyces vulgaris
Entry authors: Timofeev VI, Akparov VK, Kuranova IP

Function and Biology Details

Reaction catalysed: 
Releases a C-terminal residue, which may be hydrophobic or positively charged.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151339 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carboxypeptidase T Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 36.64 KDa
Source organism: Thermoactinomyces vulgaris
Expression system: Escherichia coli
UniProt:
  • Canonical: P29068 (Residues: 99-421; Coverage: 81%)
Gene name: cpt
Sequence domains: Zinc carboxypeptidase
Structure domains: Zn peptidases

Ligands and Environments

4 bound ligands:
Ligand ZN 1 x ZN
Ligand CA 4 x CA
Ligand SO4 2 x SO4
Ligand LU0 1 x LU0
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P6322
Unit cell:
a: 158.93Å b: 158.93Å c: 105.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.157 0.156 0.181
Expression system: Escherichia coli

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