› 6f1k

X-ray diffraction
2.2Å resolution

Structure of ARTD2/PARP2 WGR domain bound to double strand DNA without 5'phosphate

Released:
DOI: 10.2210/pdb6f1k/pdb
PDB entry 6f1k coloured by chain, front view.
PDB entry 6f1k coloured by chain, side view.
PDB entry 6f1k coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for DNA break recognition by ARTD2/PARP2.
Obaji E, Haikarainen T, Lehtiö L
Nucleic Acids Res 46 12154-12165 (2018)
PMID: 30321391

Function and Biology Details

Reaction catalysed: 
NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119869 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Poly [ADP-ribose] polymerase 2 Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 15.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4
UniProt:
  • Canonical: Q9UGN5 (Residues: 90-218; Coverage: 22%)
Gene names: ADPRT2, ADPRTL2, PARP2
Sequence domains: WGR domain
DNA (5'-D(*GP*CP*CP*TP*AP*GP*CP*TP*AP*CP*GP*TP*AP*GP*CP*TP*AP*GP*GP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 20 nucleotides
Theoretical weight: 6.13 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P6322
Unit cell:
a: 119.27Å b: 119.27Å c: 73.58Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.216 0.214 0.25
Expression systems:
  • Escherichia coli-Pichia pastoris shuttle vector pPpARG4
  • Not provided

Quick links

Citations

7 review citations

Poly(ADP-ribose) polymerase inhibition: past, present and future.
Curtin et al. (2020)
6 more

2 mentions without citation

PARP Power: A Structural Perspective on PARP1, PARP2, and PARP3 in DNA Damage Repair and Nucleosome Remodelling.
van Beek et al. (2021)
1 more