› 6f0l

Electron Microscopy
4.77Å resolution

S. cerevisiae MCM double hexamer bound to duplex DNA

Released:
DOI: 10.2210/pdb6f0l/pdb
PDB entry 6f0l coloured by chain, front view.
PDB entry 6f0l coloured by chain, side view.
PDB entry 6f0l coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Cryo-EM structure of a licensed DNA replication origin.
Abid Ali F, Douglas ME, Locke J, Pye VE, Nans A, Diffley JFX, Costa A
Nat Commun 8 2241 (2017)
PMID: 29269875
Related structures: EMD-3960

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
PDBe Complex ID:
PDB-CPX-119868 (preferred)
Entry contents:
6 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (8 distinct):
DNA replication licensing factor MCM2 Chains: 2, A
Molecule details ›
Chains: 2, A
Length: 868 amino acids
Theoretical weight: 98.91 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P29469 (Residues: 1-868; Coverage: 100%)
Gene names: MCM2, YBL023C, YBL0438
Sequence domains:
DNA replication licensing factor MCM3 Chains: 3, B
Molecule details ›
Chains: 3, B
Length: 971 amino acids
Theoretical weight: 107.65 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P24279 (Residues: 1-971; Coverage: 100%)
Gene names: MCM3, SYGP-ORF23, YEL032W
Sequence domains:
DNA replication licensing factor MCM4 Chains: 4, C
Molecule details ›
Chains: 4, C
Length: 933 amino acids
Theoretical weight: 105.14 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P30665 (Residues: 1-933; Coverage: 100%)
Gene names: CDC54, HCD21, MCM4, YP9531.13, YPR019W
Sequence domains:
Minichromosome maintenance protein 5 Chains: 5, D
Molecule details ›
Chains: 5, D
Length: 775 amino acids
Theoretical weight: 86.51 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P29496 (Residues: 1-775; Coverage: 100%)
Gene names: CDC46, L9328.1, MCM5, YLR274W
Sequence domains:
DNA replication licensing factor MCM6 Chains: 6, E
Molecule details ›
Chains: 6, E
Length: 1017 amino acids
Theoretical weight: 113.11 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P53091 (Residues: 1-1017; Coverage: 100%)
Gene names: MCM6, YGL201C
Sequence domains:
DNA replication licensing factor MCM7 Chains: 7, F
Molecule details ›
Chains: 7, F
Length: 845 amino acids
Theoretical weight: 95.05 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P38132 (Residues: 1-845; Coverage: 100%)
Gene names: CDC47, MCM7, YBR1441, YBR202W
Sequence domains:
DNA (62-MER) Chain: X
Molecule details ›
Chain: X
Length: 62 nucleotides
Theoretical weight: 19.09 KDa
DNA (62-MER) Chain: Y
Molecule details ›
Chain: Y
Length: 62 nucleotides
Theoretical weight: 19.13 KDa

Ligands and Environments

1 bound ligand:
Ligand ADP 4 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.77Å
Relevant EMDB volumes: EMD-3960
Expression system: Saccharomyces cerevisiae

Quick links

Citations

10 review citations

Mechanisms of hexameric helicases.
Fernandez et al. (2021)
9 more

7 mentions without citation

Cryo-EM structure of a licensed DNA replication origin.
Abid Ali et al. (2017)
6 more