› 6f05

X-ray diffraction
2.2Å resolution

ARABIDOPSIS THALIANA GSTF9, GSO3 BOUND

Released:
DOI: 10.2210/pdb6f05/pdb
PDB entry 6f05 coloured by chain, front view.
PDB entry 6f05 coloured by chain, side view.
PDB entry 6f05 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Redox-regulated methionine oxidation of Arabidopsis thaliana glutathione transferase Phi9 induces H-site flexibility.
Tossounian MA, Wahni K, Van Molle I, Vertommen D, Astolfi Rosado L, Messens J
Protein Sci 28 56-67 (2019)
PMID: 29732642

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131330 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase F9 Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 215 amino acids
Theoretical weight: 24.18 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: O80852 (Residues: 1-215; Coverage: 100%)
Gene names: At2g30860, F7F1.7, GLUTTR, GSTF7, GSTF9, Phi9
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GTS 10 x GTS
2 bound ligands:
Ligand GOL 4 x GOL
Ligand CL 9 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P1
Unit cell:
a: 61.43Å b: 93.97Å c: 107.71Å
α: 93.19° β: 101.57° γ: 101.97°
R-values:
R R work R free
0.164 0.162 0.209
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Physiological Roles of Plant Methionine Sulfoxide Reductases in Redox Homeostasis and Signaling.
Rey et al. (2018)
1 more

1 mention without citation

Redox-regulated methionine oxidation of Arabidopsis thaliana glutathione transferase Phi9 induces H-site flexibility.
Tossounian et al. (2019)