› 6eyf

X-ray diffraction
2.6Å resolution

Butyrylcolinesterase expressed in CHO cells co-crystallised with a rivastigmine analogue

Released:
DOI: 10.2210/pdb6eyf/pdb
PDB entry 6eyf coloured by chain, front view.
PDB entry 6eyf coloured by chain, side view.
PDB entry 6eyf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Rivastigmine and metabolite analogues with putative Alzheimer's disease-modifying properties in a Caenorhabditis elegans model
Dighe S, De la Mora E, Chan S, Kantham S, McColl G, Veliyath SK, Miles JA, Nessar ZY, Mcgeary R, Silman I, Weik I, Parat MO, Brazzolotto X, Ross B
Commun Chem (2019)

Function and Biology Details

Reaction catalysed: 
An acylcholine + H(2)O = choline + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138982 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cholinesterase Chain: A
Molecule details ›
Chain: A
Length: 527 amino acids
Theoretical weight: 59.5 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P06276 (Residues: 31-557; Coverage: 92%)
Gene names: BCHE, CHE1
Sequence domains: Carboxylesterase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

4 bound ligands:
Ligand NAG 4 x NAG
Ligand BY2 1 x BY2
Ligand GOL 4 x GOL
Ligand SO4 1 x SO4
1 modified residue:
Ligand BXT 1 x BXT

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: I422
Unit cell:
a: 154.183Å b: 154.183Å c: 127.752Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.181 0.226
Expression system: Cricetulus griseus

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