› 6eqt

X-ray diffraction
2.74Å resolution

CRYSTAL STRUCTURE OF THE HUMAN KINETOCHORE PROTEIN CENP-N

Released:
DOI: 10.2210/pdb6eqt/pdb
PDB entry 6eqt coloured by chain, front view.
PDB entry 6eqt coloured by chain, side view.
PDB entry 6eqt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Decoding the centromeric nucleosome through CENP-N.
Pentakota S, Zhou K, Smith C, Maffini S, Petrovic A, Morgan GP, Weir JR, Vetter IR, Musacchio A, Luger K
Elife 6 (2017)
PMID: 29280735

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188527 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Centromere protein N Chains: A, B
Molecule details ›
Chains: A, B
Length: 219 amino acids
Theoretical weight: 26.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96H22 (Residues: 1-213; Coverage: 63%)
Gene names: BM-309, C16orf60, CENPN, ICEN32
Sequence domains: Kinetochore protein CHL4 like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P41
Unit cell:
a: 87.3Å b: 87.3Å c: 81.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Cell organelles as targets of mammalian cadmium toxicity.
Lee et al. (2020)
10 more

8 mentions without citation

Seeing is believing: our evolving view of kinetochore structure, composition, and assembly.
Hamilton et al. (2019)
7 more