› 6ekj

X-ray diffraction
1.6Å resolution

Crystal structure of mammalian Rev7 in complex with human Chromosome alignment-maintaining phosphoprotein 1

Released:
DOI: 10.2210/pdb6ekj/pdb
PDB entry 6ekj coloured by chain, front view.
PDB entry 6ekj coloured by chain, side view.
PDB entry 6ekj coloured by chain, top view.
Source organisms:
Entry authors: Huber F, Tropia L, Emamzadah S, Halazonetis T

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188578 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitotic spindle assembly checkpoint protein MAD2B Chain: A
Molecule details ›
Chain: A
Length: 211 amino acids
Theoretical weight: 24.47 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9D752 (Residues: 1-211; Coverage: 100%)
Gene names: Mad2b, Mad2l2, Rev7
Sequence domains: HORMA domain
Chromosome alignment-maintaining phosphoprotein 1 Chain: B
Molecule details ›
Chain: B
Length: 29 amino acids
Theoretical weight: 2.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96JM3 (Residues: 328-355; Coverage: 3%)
Gene names: C13orf8, CAMP, CHAMP, CHAMP1, KIAA1802, ZNF828

Ligands and Environments

1 bound ligand:
Ligand CXS 1 x CXS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 47.26Å b: 51.62Å c: 126Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.24 0.261
Expression system: Escherichia coli BL21(DE3)

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