› 6ei4

X-ray diffraction
2Å resolution

Crystal Structure of tyrosinase from Bacillus megaterium with B5N inhibitor in the active site

Released:
DOI: 10.2210/pdb6ei4/pdb
PDB entry 6ei4 coloured by chain, front view.
PDB entry 6ei4 coloured by chain, side view.
PDB entry 6ei4 coloured by chain, top view.
Source organism: Priestia megaterium
Primary publication:
Targeting Tyrosinase: Development and Structural Insights of Novel Inhibitors Bearing Arylpiperidine and Arylpiperazine Fragments.
Ferro S, Deri B, Germanò MP, Gitto R, Ielo L, Buemi MR, Certo G, Vittorio S, Rapisarda A, Pazy Y, Fishman A, De Luca L
J Med Chem 61 3908-3917 (2018)
PMID: 29634898

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109771 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosinase copper-binding domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 288 amino acids
Theoretical weight: 33.45 KDa
Source organism: Priestia megaterium
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: B2ZB02 (Residues: 4-291; Coverage: 97%)
Sequence domains: Common central domain of tyrosinase
Structure domains: Di-copper center containing domain from catechol oxidase

Ligands and Environments

2 bound ligands:
Ligand B5N 2 x B5N
Ligand CU 4 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 53.65Å b: 78.1Å c: 81.05Å
α: 90° β: 104.52° γ: 90°
R-values:
R R work R free
0.227 0.227 0.25
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Heterocyclic Compounds as Synthetic Tyrosinase Inhibitors: Recent Advances.
Vittorio et al. (2023)
1 more

3 mentions without citation

Synthesis of Multi-Protein Complexes through Charge-Directed Sequential Activation of Tyrosine Residues.
Mogilevsky et al. (2021)
2 more