6ec0

X-ray diffraction
2.98Å resolution

Crystal structure of the wild-type heterocomplex between coil 1B domains of human intermediate filament proteins keratin 1 (KRT1) and keratin 10 (KRT10)

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-124846 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Keratin, type II cytoskeletal 1 Chain: A
Molecule details ›
Chain: A
Length: 107 amino acids
Theoretical weight: 12.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: H6VRG1 (Residues: 227-332; Coverage: 16%)
Gene name: KRT1
Sequence domains: Intermediate filament protein
Keratin, type I cytoskeletal 10 Chain: B
Molecule details ›
Chain: B
Length: 104 amino acids
Theoretical weight: 12.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13645 (Residues: 195-296; Coverage: 18%)
Gene names: KPP, KRT10
Sequence domains: Intermediate filament protein

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P3121
Unit cell:
a: 106.685Å b: 106.685Å c: 70.321Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.282 0.281 0.3
Expression system: Escherichia coli BL21(DE3)