› 6e8d

X-ray diffraction
2.34Å resolution

Crystal structure of the Bacillus subtilis sliding clamp-MutL complex.

Released:
DOI: 10.2210/pdb6e8d/pdb
PDB entry 6e8d coloured by chain, front view.
PDB entry 6e8d coloured by chain, side view.
PDB entry 6e8d coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Binding of the regulatory domain of MutL to the sliding β-clamp is species specific.
Almawi AW, Scotland MK, Randall JR, Liu L, Martin HK, Sacre L, Shen Y, Pillon MC, Simmons LA, Sutton MD, Guarné A
Nucleic Acids Res 47 4831-4842 (2019)
PMID: 30916336

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138725 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta sliding clamp; DNA mismatch repair protein MutL Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 512 amino acids
Theoretical weight: 57.36 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: P05649 (Residues: 1-378; Coverage: 100%)
  • Canonical: P49850 (Residues: 479-479, 482-574; Coverage: 15%)
Gene names: BSU00020, BSU17050, dnaG, dnaN, mutL
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08B1-1
Spacegroup: P1
Unit cell:
a: 59.02Å b: 83.89Å c: 128.26Å
α: 80.3° β: 83.57° γ: 89.96°
R-values:
R R work R free
0.216 0.216 0.25
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

RecF protein targeting to post-replication (daughter strand) gaps II: RecF interaction with replisomes.
Henry et al. (2023)
3 more

3 mentions without citation

Strand discrimination in DNA mismatch repair.
Putnam CD. (2021)
2 more