› 6du3

X-ray diffraction
2.58Å resolution

Structure of Scp1 D96N bound to REST-pS861/4 peptide

Released:
DOI: 10.2210/pdb6du3/pdb
PDB entry 6du3 coloured by chain, front view.
PDB entry 6du3 coloured by chain, side view.
PDB entry 6du3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Phosphatase activity of small C-terminal domain phosphatase 1 (SCP1) controls the stability of the key neuronal regulator RE1-silencing transcription factor (REST).
Burkholder NT, Mayfield JE, Yu X, Irani S, Arce DK, Jiang F, Matthews WL, Xue Y, Zhang YJ
J Biol Chem 293 16851-16861 (2018)
PMID: 30217818

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171547 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 180 amino acids
Theoretical weight: 20.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9GZU7 (Residues: 77-256; Coverage: 69%)
Gene names: CTDSP1, NIF3, NLIIF, SCP1
Sequence domains: NLI interacting factor-like phosphatase
Structure domains: HAD superfamily/HAD-like
RE1-silencing transcription factor Chains: C, D
Molecule details ›
Chains: C, D
Length: 12 amino acids
Theoretical weight: 1.36 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q13127 (Residues: 858-869; Coverage: 1%)
Gene names: NRSF, REST, XBR

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2
Unit cell:
a: 125.414Å b: 78.731Å c: 63.001Å
α: 90° β: 112.42° γ: 90°
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

2 review citations

News about the Role of the Transcription Factor REST in Neurons: From Physiology to Pathology.
Garcia-Manteiga et al. (2019)
1 more

1 mention without citation

Phosphatase activity of small C-terminal domain phosphatase 1 (SCP1) controls the stability of the key neuronal regulator RE1-silencing transcription factor (REST).
Burkholder et al. (2018)