› 6ciu

X-ray diffraction
1.7Å resolution

Structure of a Thr-rich interface in an Azami Green tetramer

Released:
DOI: 10.2210/pdb6ciu/pdb
PDB entry 6ciu coloured by chain, front view.
PDB entry 6ciu coloured by chain, side view.
PDB entry 6ciu coloured by chain, top view.
Source organism: Galaxea fascicularis
Primary publication:
A threonine zipper that mediates protein-protein interactions: Structure and prediction.
Oi C, Treado JD, Levine ZA, Lim CS, Knecht KM, Xiong Y, O'Hern CS, Regan L
Protein Sci 27 1969-1977 (2018)
PMID: 30198622

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178847 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Azami-Green Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 236 amino acids
Theoretical weight: 27.41 KDa
Source organism: Galaxea fascicularis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q60I25 (Residues: 2-225; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRQ 4 x CRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 70.505Å b: 86.184Å c: 141.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.165 0.205
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

The R882H substitution in the human de novo DNA methyltransferase DNMT3A disrupts allosteric regulation by the tumor supressor p53.
Sandoval et al. (2019)
1 more

1 mention without citation

A threonine zipper that mediates protein-protein interactions: Structure and prediction.
Oi et al. (2018)