› 6cbq

X-ray diffraction
2.8Å resolution

Crystal structure of QscR bound to agonist S3

Released:
DOI: 10.2210/pdb6cbq/pdb
PDB entry 6cbq coloured by chain, front view.
PDB entry 6cbq coloured by chain, side view.
PDB entry 6cbq coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Mechanism of agonism and antagonism of the Pseudomonas aeruginosa quorum sensing regulator QscR with non-native ligands.
Wysoczynski-Horita CL, Boursier ME, Hill R, Hansen K, Blackwell HE, Churchill MEA
Mol Microbiol 108 240-257 (2018)
PMID: 29437248

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193205 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH luxR-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 237 amino acids
Theoretical weight: 27.29 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RMS5 (Residues: 1-237; Coverage: 100%)
Gene names: ALP65_03645, CAZ10_26210, DT376_23495, GUL26_19290, IPC737_29575, NCTC13621_00173, PAERUG_P19_London_7_VIM_2_05_10_06810, phzR, qscR, sdiA_2
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand EVY 2 x EVY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P3121
Unit cell:
a: 94.12Å b: 94.12Å c: 105.68Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.215 0.212 0.268
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Molecular Mechanisms and Applications of N-Acyl Homoserine Lactone-Mediated Quorum Sensing in Bacteria.
Kumar et al. (2022)
2 more

5 mentions without citation

Structure-based Druggability Assessment of Anti-virulence Targets from Pseudomonas aeruginosa.
Froes et al. (2019)
4 more