› 6c7p

X-ray diffraction
2.6Å resolution

Crystal structure of D477G ACO/RPE65 chimera, monoclinic crystal form

Released:
DOI: 10.2210/pdb6c7p/pdb
PDB entry 6c7p coloured by chain, front view.
PDB entry 6c7p coloured by chain, side view.
PDB entry 6c7p coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803 substr. Kazusa
Primary publication:
Insights into the pathogenesis of dominant retinitis pigmentosa associated with a D477G mutation in RPE65.
Choi EH, Suh S, Sander CL, Hernandez CJO, Bulman ER, Khadka N, Dong Z, Shi W, Palczewski K, Kiser PD
Hum Mol Genet 27 2225-2243 (2018)
PMID: 29659842

Function and Biology Details

Reaction catalysed: 
All-trans-8'-apo-beta-carotenal + O(2) = all-trans-retinal + (2E,4E,6E)-2,6-dimethylocta-2,4,6-trienedial
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159934 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Apocarotenoid-15,15'-oxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 490 amino acids
Theoretical weight: 54.4 KDa
Source organism: Synechocystis sp. PCC 6803 substr. Kazusa
Expression system: Escherichia coli
UniProt:
  • Canonical: P74334 (Residues: 1-490; Coverage: 100%)
Gene name: sll1541
Sequence domains: Retinal pigment epithelial membrane protein

Ligands and Environments

1 bound ligand:
Ligand FE2 2 x FE2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-1
Spacegroup: C2
Unit cell:
a: 191.431Å b: 56.17Å c: 136.912Å
α: 90° β: 133.82° γ: 90°
R-values:
R R work R free
0.222 0.22 0.26
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Clinical Perspective: Treating RPE65-Associated Retinal Dystrophy.
Maguire et al. (2021)
5 more

1 mention without citation

Insights into the pathogenesis of dominant retinitis pigmentosa associated with a D477G mutation in RPE65.
Choi et al. (2018)