› 6c66

Electron Microscopy
3.66Å resolution

CRISPR RNA-guided surveillance complex, pre-nicking

Released:
DOI: 10.2210/pdb6c66/pdb
PDB entry 6c66 coloured by chain, front view.
PDB entry 6c66 coloured by chain, side view.
PDB entry 6c66 coloured by chain, top view.
Source organism: Thermobifida fusca YX
Primary publication:
Structure basis for RNA-guided DNA degradation by Cascade and Cas3.
Xiao Y, Luo M, Dolan AE, Liao M, Ke A
Science 361 (2018)
PMID: 29880725
Related structures: EMD-7347

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
PDBe Complex ID:
PDB-CPX-119652 (preferred)
Entry contents:
6 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (9 distinct):
HD Cas3-type domain-containing protein Chain: G
Molecule details ›
Chain: G
Length: 944 amino acids
Theoretical weight: 104.04 KDa
Source organism: Thermobifida fusca YX
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47PJ0 (Residues: 1-944; Coverage: 100%)
Gene name: Tfu_1593
Sequence domains:
CRISPR-associated protein, Cse1 family Chain: A
Molecule details ›
Chain: A
Length: 549 amino acids
Theoretical weight: 61.43 KDa
Source organism: Thermobifida fusca YX
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47PJ1 (Residues: 1-549; Coverage: 100%)
Gene name: Tfu_1592
Sequence domains: CRISPR-associated protein Cse1 (CRISPR_cse1)
CRISPR-associated protein, Cse4 family Chains: B, C, D, E, F, H
Molecule details ›
Chains: B, C, D, E, F, H
Length: 373 amino acids
Theoretical weight: 41.04 KDa
Source organism: Thermobifida fusca YX
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47PJ3 (Residues: 1-373; Coverage: 100%)
Gene name: Tfu_1590
Sequence domains: CT1975-like protein
CRISPR-associated protein, Cse2 family Chains: I, K
Molecule details ›
Chains: I, K
Length: 244 amino acids
Theoretical weight: 27.45 KDa
Source organism: Thermobifida fusca YX
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47PJ2 (Residues: 1-244; Coverage: 100%)
Gene name: Tfu_1591
Sequence domains: CRISPR-associated protein Cse2 (CRISPR_cse2)
CRISPR-associated protein, Cas5e family Chain: M
Molecule details ›
Chain: M
Length: 254 amino acids
Theoretical weight: 28.28 KDa
Source organism: Thermobifida fusca YX
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47PJ4 (Residues: 1-254; Coverage: 100%)
Gene name: Tfu_1589
Sequence domains: CRISPR-associated protein (Cas_Cas5)
CRISPR-associated protein, Cse3 family Chain: O
Molecule details ›
Chain: O
Length: 232 amino acids
Theoretical weight: 26.33 KDa
Source organism: Thermobifida fusca YX
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47PJ5 (Residues: 1-232; Coverage: 100%)
Gene name: Tfu_1588
Sequence domains: CRISPR associated protein
Target strand Chain: L
Molecule details ›
Chain: L
Length: 55 nucleotides
Theoretical weight: 16.8 KDa
Nontarget strand Chain: N
Molecule details ›
Chain: N
Length: 55 nucleotides
Theoretical weight: 17.1 KDa
crRNA Chain: J
Molecule details ›
Chain: J
Length: 61 nucleotides
Theoretical weight: 19.79 KDa

Ligands and Environments

1 bound ligand:
Ligand FE 2 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.66Å
Relevant EMDB volumes: EMD-7347
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Classification and Nomenclature of CRISPR-Cas Systems: Where from Here?
Makarova et al. (2018)
15 more

7 mentions without citation

Cas3 Protein-A Review of a Multi-Tasking Machine.
He et al. (2020)
6 more