› 6bqt

X-ray diffraction
2.8Å resolution

Complex of 14-3-3 theta with an IRSp53 peptide doubly-phosphorylated at T340 and T360

Released:
DOI: 10.2210/pdb6bqt/pdb
PDB entry 6bqt coloured by chain, front view.
PDB entry 6bqt coloured by chain, side view.
PDB entry 6bqt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mechanism of IRSp53 inhibition by 14-3-3.
Kast DJ, Dominguez R
Nat Commun 10 483 (2019)
PMID: 30696821

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150978 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein theta Chains: A, B, D, E, G, H, J, K
Molecule details ›
Chains: A, B, D, E, G, H, J, K
Length: 245 amino acids
Theoretical weight: 27.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27348 (Residues: 1-245; Coverage: 100%)
Gene name: YWHAQ
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
BAR/IMD domain-containing adapter protein 2 Chains: C, F, I, L
Molecule details ›
Chains: C, F, I, L
Length: 32 amino acids
Theoretical weight: 3.69 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9UQB8 (Residues: 335-366; Coverage: 6%)
Gene name: BAIAP2

Ligands and Environments

7 bound ligands:
Ligand PE4 1 x PE4
Ligand 1PE 7 x 1PE
Ligand EDO 2 x EDO
Ligand PG4 3 x PG4
Ligand GOL 2 x GOL
Ligand PGE 1 x PGE
Ligand PEG 1 x PEG
1 modified residue:
Ligand TPO 8 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER IMUS MICROFOCUS
Spacegroup: P1
Unit cell:
a: 61.103Å b: 69.572Å c: 150.473Å
α: 99.16° β: 92.98° γ: 115.98°
R-values:
R R work R free
0.236 0.234 0.287
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

Pathways to Parkinson's disease: a spotlight on 14-3-3 proteins.
Giusto et al. (2021)
2 more