› 6bpn

X-ray diffraction
2.1Å resolution

The crystal structure of the Ferric-Catecholate import receptor Fiu from E. coli K12: Open form (C2221)

Released:
DOI: 10.2210/pdb6bpn/pdb
PDB entry 6bpn coloured by chain, front view.
PDB entry 6bpn coloured by chain, side view.
PDB entry 6bpn coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
The structure of the bacterial iron-catecholate transporter Fiu suggests that it imports substrates via a two-step mechanism.
Grinter R, Lithgow T
J Biol Chem 294 19523-19534 (2019)
PMID: 31712312

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159995 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Catecholate siderophore receptor Fiu Chain: A
Molecule details ›
Chain: A
Length: 727 amino acids
Theoretical weight: 78.42 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P75780 (Residues: 34-760; Coverage: 100%)
Gene names: JW0790, b0805, fiu, ybiL
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand BOG 1 x BOG
Ligand CL 2 x CL
Ligand TRS 1 x TRS
Ligand POG 4 x POG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: C2221
Unit cell:
a: 107.927Å b: 148.945Å c: 105.061Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.175 0.22
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Iron Acquisition by Bacterial Pathogens: Beyond Tris-Catecholate Complexes.
Zhang et al. (2020)
1 more

1 mention without citation

Iron Acquisition Systems of Gram-negative Bacterial Pathogens Define TonB-Dependent Pathways to Novel Antibiotics.
Klebba et al. (2021)